Develosil HSR AQ C18 HSR C1 HPLC Columns INDIA
The Develosil AQ HSR C18 and Develosil HSR C1 help you separate high polarity compounds on a reverse phase column. The Develosil HSR AQ C18 and Develosil HSR C1 gives you maximum performance even in 100% aqueous mobile phase conditions.
As being part of the HSR series, these columns are subjected to original end cap technology to maximize peak shape in acidic or basic conditions.
Develosil HSR AQ C18 and HSR C1 Specifications
| Column | Ligand | Carbon | End Capping | Surface Area | Pore Diameter | Range of pH | Particle Size |
|---|---|---|---|---|---|---|---|
| HSR AQ C18 | ODS/C18 | 18% | Yes | 450 m2/g | 100 Å | pH 2-9 | 2 µm, 3 µm, 5 µm |
| HSR C1 | TMS/C1 | 6.5% | pH 2-8 | 3 µm, 5 µm |
Featured Applications
Analysis of Water Soluble Vitamins
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil HSR AQ C18, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | A) 25mM NH₄H₂PO₄ (pH=3.0) B) Methanol |
| Gradient: | B) 5%(0-2min), 5%-50%(2-7min), 50%(7-10min), 5%(10.1min-) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 210 nm |
| Sample: | 1. Thiamin HCl (0.48mg/mL) 2. L(+)-Ascorbic acid (0.49mg/mL) 3. Nicotinic acid (0.49mg/mL) 4. Pyridoxine HCl (0.46mg/mL) 5. Nicotinamide (0.50mg/mL) 6. Calcium (+)-pantothenate (0.65mg/mL) 7. Cyanocobalamin (0.46mg/mL) 8. Folic acid (0.46mg/mL) 9. Riboflavin sodium phosphate (0.46mg/mL) 10. (+)-Biotin (0.47mg/mL) |
| Injection Volume: | 1.0 µL |
Analysis of Catechin in Green Tea
| HPLC Analytical Conditions: | |
|---|---|
| Column: | 1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
2. Develosil HSR C1, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | Methanol/25mM NH₄H₂PO₄ (pH=2.0)=25/75 |
| Gradient: | B) 5%(0-2min), 5%-50%(2-7min), 50%(7-10min), 5%(10.1min-) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 280 nm |
| Sample: | 1. (-)-Epigallocatechin (0.25mg/mL) 2. (-)-Epigallocatechin gallate (0.30mg/mL) 3. Caffeine 4. (-)-Epicatechin (0.25mg/mL) 5. (-)-Epicatechin gallate (0.30mg/mL) |
| Injection Volume: | 2.0 µL |
Analysis of Peptides
HPLC Analytical Conditions: | |
|---|---|
| Column: | 1. Develosil HSR C1, 5 µm (4.6 x 150 mm) 2. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | A. 25 mM NaCl (pH 3.0) B) Acetonitrile |
| Flow Rate: | 1.0 mL/min |
| Gradient: | See more data here. |
| Temperature | 30°C |
| Sample: | 1.Gly-Tyr (0.05mg/mL) 2.Val-Tyr-Val (0.05mg/mL) 3.Met-Enkephalin (0.05mg/mL) 4.Angiotensin II (0.05mg/mL) 5.Leu-Enkephalin (0.05mg/mL) |
| Injection Volume: | 10.0 µL |
