Develosil HSR AQ C18 HSR C1 HPLC Columns INDIA

The Develosil AQ HSR C18 and Develosil HSR C1 help you separate high polarity compounds on a reverse phase column. The Develosil HSR AQ C18 and Develosil HSR C1 gives you maximum performance even in 100% aqueous mobile phase conditions.

As being part of the HSR series, these columns are subjected to original end cap technology to maximize peak shape in acidic or basic conditions.

Develosil HSR AQ C18 and HSR C1 Specifications

 

ColumnLigandCarbonEnd CappingSurface AreaPore DiameterRange of pHParticle Size
HSR AQ C18ODS/C1818%Yes450 m2/g100 ÅpH 2-92 µm, 3 µm, 5 µm
HSR C1TMS/C16.5%pH 2-8

3 µm, 5 µm

Featured Applications

Analysis of Water Soluble Vitamins

HPLC Analytical Conditions:
Column: Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
Mobile Phase:A) 25mM NH₄H₂PO₄ (pH=3.0)
B)  Methanol
Gradient:B) 5%(0-2min), 5%-50%(2-7min), 50%(7-10min), 5%(10.1min-)​
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 210 nm
Sample:1. Thiamin HCl (0.48mg/mL)
2. L(+)-Ascorbic acid (0.49mg/mL)
3. Nicotinic acid (0.49mg/mL)
4. Pyridoxine HCl (0.46mg/mL)
5. Nicotinamide (0.50mg/mL)
6. Calcium (+)-pantothenate (0.65mg/mL)
7. Cyanocobalamin (0.46mg/mL)
8. Folic acid (0.46mg/mL)
9. Riboflavin sodium phosphate (0.46mg/mL)
10. (+)-Biotin (0.47mg/mL)
Injection Volume:1.0 µL

Analysis of Catechin in Green Tea

HPLC Analytical Conditions:
Column: 1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)

 

2. Develosil HSR C1, 5 µm (4.6 x 150 mm)

Mobile Phase:Methanol/25mM NH₄H₂PO₄ (pH=2.0)=25/75
Gradient:B) 5%(0-2min), 5%-50%(2-7min), 50%(7-10min), 5%(10.1min-)​
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 280 nm
Sample:1. (-)-Epigallocatechin (0.25mg/mL)
2. (-)-Epigallocatechin gallate (0.30mg/mL)
3. Caffeine
4. (-)-Epicatechin (0.25mg/mL)
5. (-)-Epicatechin gallate (0.30mg/mL)
Injection Volume:2.0 µL

Analysis of Peptides

HPLC Analytical Conditions:

Column: 1. Develosil HSR C1, 5 µm (4.6 x 150 mm)
2. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
Mobile Phase:A. 25 mM NaCl (pH 3.0)
B) Acetonitrile
Flow Rate:1.0 mL/min
Gradient:See more data here.
Temperature30°C
Sample:1.Gly-Tyr (0.05mg/mL)
2.Val-Tyr-Val (0.05mg/mL)
3.Met-Enkephalin (0.05mg/mL)
4.Angiotensin II (0.05mg/mL)
5.Leu-Enkephalin (0.05mg/mL)
Injection Volume: 10.0 µL

 

Develosil HSR AQ C18 HSR C1 HPLC Columns INDIA

Develosil HSR AQ C18 HSR C1 HPLC Columns INDIA