Ultra-high resolution like sub-2μm columns
Faster separation like sub-2μm columns
Lower back pressure than sub-2μm columns resolve chemically similar analytes.
Compatible with exsisting HPLC or LC/MS.
Vertical UPS HPLC COLUMN Vertical UPS HPLC COLUMN

 

    VertiSep™ Integral columns are made with superficially porous silica microsphere (Core-shell) technolohy. The packings consist of ultra-pure silica in solid cores and thin outer shells with uniform pores. These offer excellent kinetic properties providing stable, ultra-high resolution and faster than HPLC column with conventional packings.

    VertiSep™ Integral columns are 2.6μm in particle size, very narrow particle size distribution and the higher particle density. This provides efficiency of about at least 20,000 in which comparable to sub-2μm columns.

    VertiSep™ Integral particles have a solid core of 1.7μm and 450nm thick outer shell respectively. The thin outer porous shell allows rapid solute mass transfer (fast kinetics) so that mobile phase flow rate can be increased to allows for very fast separations without sacrificing resolution like sub-2μm columns.

    As only the thin outer shell of core shell particle is porous. This allows less back pressure and rapid solute diffusion in and out of the thin porous shell comparing to totally porous sub-2μm particle. VertiSep™ Integral columns limit at pressure up to at least 5,000psi that are compatible with standard HPLC and LC/MS equipments.

     All totally porous particles (1.8μm, 3μm, and 5μm) have an acceptable particle size distribution. However, VertiSep™ Integral core- shell particle has a narrow particle size distribution, which reduces multi-path diffusion and results in high efficiency.

 

Vertical UPS HPLC COLUMN

Vertical UPS HPLC COLUMN
Column : 3.0 x 100mm
Mobile phase : Acetonitrile/water
Mobile phase : (60/40)
Flow rate : 0.5mL/min
Injection : 3μL
Temperature : 25 ℃
Detection : UV 254nm
Analytes : Toluene

The Van Deemter shows that the smaller (2.6μm) particle size of VertiSep™ Integral particles results in signifi cantly higher effi ciency for toluene than 5μm and 3μm particle columns. The less increase in plate height with mobile phase velocity increase for the VertiSep™ Integral particles is also in keeping with the smaller particle size and the high kinetic properties of core shell structure.
In comparison with sub-2μm column, VertiSep™ Integral shows very similar effi ciency; however, but less backpressures that fl ow rates can be increased to speed up analysis.

Vertical UPS HPLC COLUMN
Column Column : 3.0 x 100mm
Mobile phase : Acetonitrile/water
Mobile phase : (60/40)
Flow rate : 0.5mL/min
Injection : 3μL
Temperature : 25 ℃
Detection : UV 254nm
Analytes :
1 Uracil
2 Phenol
3 N,N-Diethyl-m-Toluamide
4 Toluene

The effi ciency on VertiSep™ Integral column is about more than 90% of sub-2μm columns and about 2 times the effi ency of the 3μm columns.
The pressure on VertiSep™ Integral column is below 5,000psi while the pressure on sub-2μm column is above 6,000psi. VertiSep™ Integral columns are compatible with all HPLC systems.

VertiSep™ pHendure HPLC Columns
 Practical Extreme pH in RP-HPLC
 Using Hybrid Particle Based Packings
     Introduction
     Spherical hybrid organic-inorganic particles combine the high efficiency and excellent mechanical strength of silica with the wide pH stability range and reduced silanol effects of polymers. Reversed-phase HPLC packing based on hybrid particles exhibits excellent efficiency, selectivity and long lifetimes at alevated temperatures.
    The problem of extreme pH in silica column
    Silica-based materials are the most widely used packing for RP-HPLC due to the high efficiency and excellent mechanical strength. However, there are some limitations on these conventional silica-base materials.
     Short lifetimes below pH 2 (hydrolysis of bonded phase)
     Short lifetimes below pH 8 (dissolution of silica)
     Broad, tailing peaks for basic compounds
     Short life time at high temperatures
     Why operate outside pH2-8
    The mobile phase pH is an important factor in retention, selectivity andeffciency for polar compounds. pH also influences analyte ionisation, which is necessary for LC-MS. Finally, the stability of the analyte may be improved at high or low pH. A good column for low and high pH should offers acceptable lifetime, no bleed, stable retention and effciency.
     What is Hybrid particle based packings ?
    The Hybrid particle contains both silica and organosiloxane and shares the advantages of both. The Hybrid particle is prepared by solgel synthesis using organoslanes. This particle is as hard assilica and does not well or shrink in the presence of organic solvents. The pH stability of bonded phases based on this Hybrid particle exceeds that of silica-based bonded phases.
Vertical UPS HPLC COLUMN
   .What is VertiSep™ pHendure
     The new VertiSep™ pHendure from Vertical Chromatography is a Hybrid organic-inorganic particle based packings providing extended pH Stability from 1-12, a novel solution to the problems exhibited by conventional solica-based and polymer-based packing material.
     What are the features ?
     Long lifetimes below pH 2 (Low pH stability)
     Long lifetimes above pH 8 (high pH stability)
     Long life time at high temperatures(stability of hybrid particle)
     Low bleed, ideal for both LC-MS and routine HPLC method development (stability of hybrid particle)
     VertiSep™ pHendure HPLC columns are packed with hybrid silica organosilane particle based packings providing pH stability pH 1-12 and high resolution, a novel solution to the problems on acidic or basic conditions exhibited by conventional silica-based columns.
     Hydrolysis of bonded phase will occur and causes loss of efficiency under acidic conditons with conventional silica-based column. VertiSep™ pHendure with the hybrid particle based packings solves this problem and provides excellent stability under acidic conditions offerring long lifetime.
     Dissolution of silica will occurs and causes tailing peaks under basic conditions with conventional silica-based column. VertiSep pHendure with the hybrid particle based packings solves this problem and provides excellent stability under basic conditions offering symmetrical peaks.
     VertiSep™ pHendure is low bleed, ideal for both LC-MS and routine HPLC method development.
     VertiSep™ pHendure C18 is 14% carbon loading offering higher capacity and greater resolution for all HPLC applications including USP L1.
     VertiSep™ pHendure Phenyl-Hexyl is 12% carbon loading offering an alternative selectivity to C18 for analytes containing aromatic rings or USP L11..
Vertical UPS HPLC COLUMN

Stability under acidic and high temperature conditions

Vertical UPS HPLC COLUMN



VertiSep™ pHendure C18

Hybrid Particle columns were exposed to 1.5% Trifl uoroacetic acid in ACN/Water at pH 1.0 at 60 ℃ for 100 hours at a fl ow rate of 1mL/min, then tested the columns with a standardized test condition and Napthalene as test probe. VertiSep™ pHendure hybrid particle exhibits excellent stability under acidic conditions better than conventional silica-based packings
.

Stability under basic conditions

Vertical UPS HPLC COLUMN

 

VertiSep™ pHendure C18
Hybrid Particle columns were exposed to 0.2% Triethylamine in ACN/Water at pH 11.5 at 30 ℃ for 100 hours at a fl ow rate of 1mL/min, then tested the columns with a standardized test condition and amitriptyline as test probe. VertiSep™ pHendure hybrid particle exhibits excellent stability and provides symmetrical peak for basic compounds under basic conditions better than conventional silica-based packin

Ultra performance silica with excellent stability, efficiency and column-to-column reproducibility
•Ultra-high surface area offer ultra high resolution for gradient elution of difficulty separation compounds
•Fully endcapped to improve peak shape
 
•Monomeric bonding offers low back pressure and high column efficiency to better resolve chemically similar analytes.
     VertiSep™ UPS packings are ultra-high purity spherical silica which contains less amounts of metal ions and sulfate contaminants offerring ultra performance with excellent stability at pH 1.5-10.0*, excellent efficiency, column-to-column reproducibility, long lifetime and ideal for LC/MS or ELSD applications.

     VertiSep™ UPS packings are ultra-high surface of 450 m2/g offer greater capacity and higher resolution for gradient elution of difficult separation compounds like basic drugs, organic acids and other polar compounds.

     VertiSep™ UPS reverse phase packings are fully endcapped after bonding to cover unreached silanols and improve peak symmetry for basic analytes.

     VertiSep™ UPS packings are monomeric bonding resulting in lower column back pressure and high column efficiency to better resolve chemically similar analytes.

     VertiSep™ UPS are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor.

Vertical UPS HPLC COLUMN

     VertiSep™ UPS C8 offers the highly degree of hydrophobicity for pharmaceuticals, nucleotides and polar compounds.

     VertiSep™ UPS Ph is a reverse phase chemistry offering high hydrolytic stability for high hydrophobic compounds.

     VertiSep™ UPS CN can be used both reverse phase or normal phase offfering excellent reproducibility and performance for polar compounds in accordance with USP L10.

     VertiSep™ UPS Si is normal phase packings offering excellent reproducibility and performance for polar compounds in accordance with USP L3.

     VertiSep™ UPS HILIC offers highly degree of retention for polar compounds in a highly organic mobile phase.

     VertiSep™ UPS PFP offers multiple mechanisms such as Hydrogen Bonding, Dipole-Dipole Interactions, Aromatic Π – Π Interactions and Hydrophobic for alternative selectivity.

Vertical UPS HPLC COLUMN

Ultra-high purity spherical silica offer excellent stability, efficiency and column-to-column reproducibility
•Stable and strong retention in 100% aqueous mobile phase for hydrophilic or polar compounds
•High carbon loading C18 offers high degree of hydrophobicity
•High surface area offers high resolution for gradient elution of diffculty separation compounds
•Fully endcapped to improve peak shape

     VertiSep™ AQS packings are ultra-high purity silica which contains less amounts of metal ions and sulfate contaminants offer excellent stability, efficiency, column-to-column reproducibility and long lifetime at high and low pH levels at pH 1.5-10.0* and ideal for LC/
MS or ELSD application.

     VertiSep™ AQS packings are stable and strong retention in 100% aqueous mobile phase for hydrophilic or polar compounds such as biomolecules, metabolytes, oligosaccharides, amino acids, small peptides, nucleotides and organic acids.

     VertiSep™ AQS packings are high surface of 300 m2/g offer high capacity and high resolution for fast gradient elution.

 Monomeric bonding offers low back pressure and high column efficiency to better resolve chemically similar analytes
Vertical UPS HPLC COLUMN

    VertiSep™ AQS reverse phase C18 packings are high carbon loading of 15% offerring the high degree of hydrophobicity.

     VertiSep™ AQS reverse phase packings are fully endcapped an show selectivity as conventional C18 phases.

     VertiSep™ AQS packings are monomeric bonding resulting in lower column back pressure and high column efficiency to better resolve chemically similar analytes.

     VertiSep™ AQS are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor.

Vertical UPS HPLC COLUMN

High purity spherical silica offers high stability, efficiency and column-to-column reproducibility
Highest carbon loading C18 offers highest degree of hydrophobicity for highest resolution of organic compounds that have similar structures
High surface area offers high resolution for gradient elution
High surface area offers high resolution for gradient elution of diffculty separation compounds
Fully endcapped to improve peak symmetry
Polymeric bonding offers high stability and longer column lifetime

     VertiSep™ HCS packings are high purity silica which contains less amonts of metal ions and sulfate contaminants offer high stability at pH 1.5-10.0* efficiency, column-to-column reproducibility and long lifetime.

     VertiSep™ HCS reverse phase C18 packings are highest carbon loading of 23% offerring the highest degree of hydrophobicity for highest resolution of organic compounds that have similar structures such as polynuclear aromatic hydrocarbons, benzidines, vitamins D2, D3, PTH and amino acids.

     VertiSep™ HCS packings are high surface of 350 m2/g offer high capacity and high resolution for gradient elution.

     VertiSep™ HCS reverse phase packings are fully endcapped and show selectivity as conventional C18 phases.

     VertiSep™ HCS packings are polymeric bonding resulting high stability and longer column lifetime.

     VertiSep™ HCS are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor.

Vertical UPS HPLC COLUMN
An exellent alternative to Hypersil® BDS at a reasonable lower price
Most methods on Hypersil® BDS can be transfered to VertiSep™ BDS
Excellent Column-to-Column reproducibility
Full guarantee

Vertical UPS HPLC COLUMN

     VertiSep™ BDS are highly base deactivation for basic compounds while offer superior efficiency for neutral and acidic compounds. VertiSep™ BDS has four stable reverse phase packings C18, C8, Phenyl and Cyano that well suited for pharmaceutical and other biological compounds

     VertiSep™ BDS was developed to provide a physical and performance characteristics very similar to those of Hypersil® BDS.

    VertiSep™ BDS provides the results indicated comparable capacity, selectivity efficiency and peak symmetry when compared to Hypersil® BDS. Most methods developed on Hypersil® BDS can be transfered to VertiSep™ BDS, even for USP applications.

 

Vertical UPS HPLC COLUMN

 

     VertiSep™ BDS are manufactured to minimize lot-to-lot variation with a strict QC program to ensure excellent column-to-column reproducibility.

     VertiSep™ BDS are guaranteed separations of your samples will be comparable to separations with Hypersil® BDS columns. If VertiSep™ BDS does not meet your satisfaction, send in comparative data within 45 days and keep VertiSepTM BDS column for FREE.

     VertiSep™ BDS are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor.

 

Vertical UPS HPLC COLUMN
High purity base-deactivated silica for sharp symmetrical peaks
Dual retention mechanism; hydrophobic and hydrophilic with enhanced-polar selectivity
Monomeric bonding offers low back pressure and high column efficiency to better resolve chemically similar analytes.
Exceptional efficiency and reproducibilty

     VertiSep™ EPS are high purity spherical base-deactivated silica which contains less amounts of metal ions and sulfate contaminants offerring ultra performance with excellent stability at pH 1.5-7.5 and symmetrical peak shape for sensitive metal compounds.

     VertiSep™ EPS are monomeric bonding offers low back pressure and high column efficiency to better resolve chemically similar analytes.

Vertical UPS HPLC COLUMN

     VertiSep™ EPS have both hydrophobic and hydrophilic selectivity. The polar funtional group of the compounds interact secondarily with hydrophilic site and are retained primarily by reversed phase mechanism. This special design offers unique selectivity for separation of polar compounds containg amine, carboxyl, phenol, cyano, hydroxide, nitrite, brominated, fluorinate and chlorinated groups found in pharmacutical, food and environmental applications.

     VertiSep™ EPS are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor

Vertical UPS HPLC COLUMN

High purity silica offer high stability, efficiency, reproducibility
Moderate pore size and surface area are perfect for general purpose
High carbon loading C18 offers high degree of hydrophobicity for high resolution of organic compounds that have similar structures
Full guaranteeFully endcapped to improve peak symmetry
Polymeric bonding offers high stability and longer column lifetime
Economic Price

     VertiSep™ GES packings are high purity silica which contains less amounts of metal ions and sulfate contaminants offer high stability at pH 1.5-10.0*, efficiency, column-to-column reproducibility.

     VertiSep™ GES packings are moderate pore size and surface area proving general purpose separations of variety of compounds.

     VertiSep™ GES reverse phase C18 packings are high carbon loading of 17% offerring the high degree of hydrophobicity for high resolution of organic compounds that have similar structures such as polynuclear aromatic hydrocarbons, benzidines, vitamins D2, D3, amino acids.

     VertiSep™ GES reverse phase packings are fully endcapped an show selectivity as conventional C18 phases.

     VertiSep™ GES packings are polymeric bonding resulting high stability and longer column lifetime.

     VertiSep™ GES are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor

Vertical UPS HPLC COLUMN

Vertical UPS HPLC COLUMN

Full endcapping
Ultimate protein and peptide application column
Acidic and alkalic resistance for long lifetime
4 Phases: C30, C18, C8, and C4 chemistries
3mm, 5mm, 10mm particle sizes

     VertiSep™ BIO packings are ultra-high purity spherical silica, full endcapping providing separation or purification of high molecular weight compounds like protein and peptide.

     Because of the significant improvement of acidic and alkalic resistance, it can be use for extened period of time in acidic mobile phase condition and rinsed for recover with NaOH containing buffer. There are 4 phases: C30, C18, C8 and C4 and 3 particle sizes: 3µm, 5µm, 10µm particle sizes.

     VertiSep™ BIO are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor

Vertical UPS HPLC COLUMN

     VertiSep™ BIO C30 are ideal for the separation of large molecules such as proteins and peptides, especially isomers in the carotenoid family of long chain molecules. VertiSep™ BIO C30 are a great equivalent to YMC C30 and are interchangeable with them for many applications.

     VertiSep™ BIO C18 are ideal for the separation of high molecular weight peptides and oligonucleic acids. VertiSep™ BIO C18 are designed for biopharmaceutical, biochemical and environmental applications and for separation of food compounds as well.

     VertiSep™ BIO C8 have moderate hydrophobicity make them useful for separating compounds or samples in cases where separation optimization is difficult to achieve using C18 with relatively high hydrophobicity. VertiSep™ BIO C8 are used to separate many classes of compounds including pharmaceuticals, organic chemicals and biologicals.

     VertiSep™ BIO C4 stationary phase surface hydrophobicity is lower than both C18 and C8. VertiSep™ BIO C4 achieve better separation than C18 or C8 for some type of samples. VertiSep™ BIO C4 effectively resolve many classes of proteins and biopolymers.

Vertical UPS HPLC COLUMN

An excellent alternative to Waters® mBondapakat a resonable lower price
Most methods on mBondapakcan be transfered to VertiSepIRS
Excellent Column-to-Column reproducibility
Full guarantee

     VertiSep™ IRS packings were developed to provide a physical and performance characteristics very similar to those of Waters® µBondapak™.

     VertiSep™ IRS packings provide the results indicated comparable capacity, selectivity efficiency and peak symmetry when compared to ?Bondapak™. Most methods developed on ?BondapakTM can be transfered to VertiSep™ IRS, even for USP applications.

     VertiSep™ IRS packings are manufactured to minimize lot-to-lot variation with a strict QC program to ensure excellent column-tocolumn reproducibility.

     VertiSep™ IRS packings are guaranteed separations of your samples will be comparable to separations with µBondapak™ columns. If VertiSep™ IRS does not meet your satisfaction, send in comparative data within 45 days and keep VertiSep™ IRS column for FREE.

Vertical UPS HPLC COLUMN

     VertiSep™ IRS are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor.

Vertical UPS HPLC COLUMN

Multifunctional silica based column
Used in specific applications and recommended by USP
Excellent Column-to-Column reproducibilityHigh surface area offers high resolution for gradient elution of difficulty separation compounds
Polymeric bonding offers high stability and longer column life time
Excellent Column-to-Column reproducibility
Available in 5 phases : CN/NH2, C8/NH2, C8/C18, C8/SCX, C8/SAX and Ph/C6
Vertical UPS HPLC COLUMN

     VertiSep™ MMS columns are multifunctional silica based columns used as alternative to single phase for specific applications. VertiSepTM MMS are high surface of 450 m2/g offer high capacity and high resolution for gradient elution. VertiSep™ MMS columns are manufactured to minimize lot-to-lot variation with a strict QC program to ensure excellent column-to-column reproducibility.

     VertiSep™ MMS CN/NH2 is a multifunctional phases of cyano and amino offering reversed phase, normal phase and weak anion exchange interactions for good thermal and chemical stability. Selectivity and rapid equilibrium allow a range of separation mechanisms to be used, including adsorption, reversed phase and weak anion exchange depending on mobile phase used. VertiSep™ MMS CN/NH2 is a good choice for carbohydrate separations and recommended for USP L18.

     VertiSep™ MMS C8/NH2 column is a multifunctional phase of C8 and amine offering both reversed phase and normal phase interaction. VertiSep™ MMS C8/NH2 is used for difficult applications i.e. hydrophobic as well as ionic compounds which previously required special conditions can be developed by controlling ionization via mobile phase adjustments, buffer strength and organic modifier composition. VertiSep™ MMS C8/NH2 is recommended for USP L28.

     VertiSep™ MMS C8/C18 column is multifunctional phase of C8 and C18 groups for the reversed phase separation of basic molecules. VertiSep™ MMS C8/C18 offers more excellent gradient separation to C18 or C8 only for applications especially pharmaceutical applications and recommended for USP L42.

     VertiSep™ MMS C8/SCX is a multifunctional phase of C8 and SCX (Strong Cationic Exchanger) offering both reversed phase and strong cationic interactions. VertiSep™ MMS C8/SCX is used for separation of basic drugs and metabolites from physiological fluids. VertiSep™ MMS C8/SCX is recommended for USP L44. Difficult applications which previously required special conditions can be developed by controlling ionization via mobile phase adjustments, buffer strength and organic modifier composition.

     VertiSep™ MMS C8/SAX is multifunctional phase of C8 and SAX (Strong Anionic Exchanger) offering both reversed phase and strong anionic interactions. VertiSep™ MMS C8/SAX is used for acidic drugs and metabolites from physiological fluids. Difficult applications which previously required special conditions can be developed by controlling
ionization via mobile phase adjustments, buffer strength and organic modifier composition.

     VertiSep™ MMS Ph/C6 is multifunctional phase of phenyl and hexyl (C6) offering both phenyl and a short alkyl phase (C5 or C8) interactions, dense bonding and endcapping to minimize silanol effect and increases chemical stability. VertiSep™ MMS PH/C6 offers dual selectivity of phenyl ringe and hexyl chain for aromatic and amine compounds and recommended for USP L11.

     VertiSep™ MMS are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor.

High purity silica for high stability, efficiency, and reproducibility
Smal pore size and surface area, perfect for general purpose and method development
Fully endcapped to improve peak symmetry
Polymeric bonding offerring high stability at pH range 1.5 – 10 and longer column lifetime
Direct replacement to Waters® Spherisorb® and Agilent® Zorbax® Eclipse XDB

     VertiSep™ SPS packings are high purity silica which contains less amounts of metal ions and sulfate contaminants offerrring high stability, efficiency, column-to-column reproducibility.

     VertiSep™ SPS packings are small pore size and surface area proving general purpose separations of variety of smale compounds and also method developement.

     VertiSep™ SPS reverse phase packings are fully endcapped and shows selectivity as conventional C18 phases.

     VertiSep™ SPS packings are polymeric bonding resulting high stability at pH 1.5-10.0* and longer column lifetime.

Vertical UPS HPLC COLUMN

     VertiSep™ SPS packings provide the results indicated comparable capacity, selectivity efficiency and peak symmetry when compared to Waters® Spherisorb® and Agilent® Zorbax® Eclipse XDB. Almost methods developed on those can be transfered to VertiSep™ SPS, even for USP applications.

     VertiSep™ SPS packings are manufactured to minimize lot-tolot variation with a strict QC program to ensure excellent column-tocolumn reproducibility.

     VertiSep™ SPS packings are guaranteed separations of your samples will be comparable to separations with Waters® Spherisorb® or Agilent® Zorbax® Eclipse XDB columns. If VertiSep™ SPS does not meet your satisfaction, send in comparative data within 45 days and keep VertiSep™ SPS column for FREE.

     VertiSep™ SPS are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor.

Vertical UPS HPLC COLUMN
Polymer based column
Wide pH stability
Excellent efficiency and resolution
Reproducibility lot-to-lot and column-to-column
Use only water as mobile phase
Vertical UPS HPLC COLUMN

     VertiSep™ SUGAR CMP columns contain 8% cross-linked spherical Polystyrene Divinylbenzen (PS-DVB) polymer with calcium ionic form. Available in 9 ?m particle size. VertiSep™ SUGAR CMP columns are useful for analysis of mono-, disaccharides and sugar alcohols by only water as the mobile phase. The dimension of 4.0×250 mm is recommended for USP L-19 for separation of sugar alcohols such as sorbitol and manitol. Typical applications include fruit juices, soft drinks, dairy products, vegetables and medical source.

     VertiSep™ SUGAR SOP columns contain 8% cross-linked spherical Polystyrene Divinylbenzen (PS-DVB) polymer with silver ionic form. Available in 10 and 20 ?m particle size. They provide rapid oligosaccharides separation. Particle size of 10 ?m can resolve saccharides as large as DP-7 and the 20 ?m can resolve saccharides as large as DP-12. VertiSep™ SUGAR SOP columns are also useful in the carbohydrate industry to determine hydrolyzates in the conversion of corn syrup to fermentable carbohydrates.

     VertiSep™ SUGAR LMP columns contain 8% cross-linked spherical Pylystyrene Divinylbenzene (PS-DVB) polymer with lead ionic form. Available in 8 ?m particle size. They provide highest resolution and selectivity for monosccharides and disaccharides. VertiSep™ SUGAR LMP columns also can resolve pentoses and hexoses found in cellulose products especially glucose, xylose, galactose, cellobiose, arabinose and mannose which are not completely resolved on the calcium form. In addition, VertiSep™ SUGAR LMP columns can resolve sucrose and lactose well if these two sugars are present in excess in some samples. Typical applications include dairy and meat industries, cereals and plant fibers.

     VertiSep™ SUGAR HMP columns contain 8% cross-linked spherical Polystyrene Divinylbenzene (PS-DVB) polymer with hydrogen ionic form. Available in 8 ?m particle size. They are useful for analysis of samples containing monosaccharides in combination with organic acids, fatty acids and alcohols by using only a dilute sulfuric acid as mobile phase at ambient temperatures. Typical applications include wine industries, dairy industries, bio-reactions and medical science.

     VertiSep™ SUGAR are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor.

pH stable range 1-14
Excellent analyte recovery
Very rugged, long lasting materials
Great peak shape for all types on compounds
Good reproducibility from column to column
VertiSep PRP HPLC COLUMN

     VertiSep™ PRP-L21 HPLC columns are 100% Polystyrene Divinylbenzene (PS-DVB) for compatibility with equivalent high efficiency as silica-based C18 columns and pH stable range 1-14. VertiSep™ PRP-L21 columns comply with USP L21 specification and ideal for reverse phase separation of biopharmaceutical and pharmaceutical applications.

     VertiSep™ PRP-AQ HPLC columns are Hydoxylated Polystyrene Divinylbenzene (PS-DVB-OH) for difference selectivity by reverse phase or normal phase chromatography. VertiSep™ PRP-AQ columns can be use with 100% water with minimum shrinkage. VertiSep™ PRP-AQ columns are ideal for polar compounds.

     VertiSep™ PRP-C18 HPLC columns are Polyvinylaclohol-based C18 for replacement of silica-based C18 columns due to the same separation efficiency and logical elution order. VertiSep™ PRP-C18 columns are stable in pH range 2-13 solutions not possible on silica. Polyvinylalcohol offers minimum shrinkage and swelling. VertiSep™ PRP-C18 columns are ideal for small analytes, peptides and small proteins.

     VertiSep™ PRP-C8 HPLC columns are polyvinylaclohol-based C8 for replacement of silica-based C8 columns due to the same separation efficiency and logical elution order. VertiSep™ PRP-C8 columns are stable in pH range 2-13 solutions not possible on silica. Polyvinylalcohol offers minimum shrinkage and swelling. VertiSep™ PRP-C8 columns are ideal for small analytes, peptides and small proteins.

     VertiSep™ PRP-NH2 HPLC columns are polyvinylalcohol-based NH2 for replacement of silica-based NH2 columns due to the same separation efficiency and logical elution order. VertiSep™ PRP-NH2 columns are stable in pH range 2-13 solutions not possible on silica. Polyvinylalcohol offers minimum shrinkage and swelling. VertiSep™ PRP-NH2 columns are ideal for analysis of saccharides in normal phase like silica-based NH2 columns but longer life-time.

     VertiSep™ PRP are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor

Provide symetrical peak shapes for inoganic and organic anions by both Suppressed and Nonsuppressed IC
Compatible with common mobile phases
Separate fluoride away from the water dip

        VertiSepAnion AX1 column is packed with hydrophilic copolymer with quaternary ammonium functional group providing symmetrical peak shapes for inoganic and organic anions by both Suppressed and Nonsuppressed IC.

VertiSepAnion AX1 column is compatible with common mobile phases: p-hydroxybenzoate, sodium hydroxide, and sodium carbonate/bicarbonate. The p-hydroxybenzoate mobile phase is best used for bicarbonate and the seven common inorganic anions. Sodium hydroxide mobile phasweak acid anions.

VertiSep PRP HPLC COLUMN
Separates Common Anions and Strongly Retained Hydrophobic Anions
Compatible with common mobile hasesSeparates weak organic acids anions
Hydrophilic Packing for Maximum Efficiency
Ideal for Gradient Separations with Carbonate/Bicarbonate Mobile Phases

       VertiSepAnion AX2 column is packed with hydrophilic copolymer with quaternary ammonium functional groups providing symmetrical peak shapes for all anions by Suppressed IC.

VertiSepAnion AX2 column is ideal for routine common anions analysis and for determining other strongly retained hydrophobic anions such as iodide, thiosulfate, and thiocyanate. Carbonate/bicarbonate gradients offer a powerful tool for separating anions that have a wide range of affinities for the column.

VertiSepAnion AX2 column can also be used to determine weak organic anions such as acetate and formate without the interference from fluoride and chloride.

VertiSep PRP HPLC COLUMN
Meets the Requirements of US EPA Methods 300.1, 300.0, and 317.0.
Separates Common Inorganic Anions and Oxyhalide Anions
New 5μm resin particle impoves efficiency

       VertiSep™ Anion AX300 column is packed with hydrophilic copolymer with quaternary ammonium functional groups providing symmetrical peak shapes for all anions by Suppressed IC.

VertiSep™ Anion AX300 column ideals for separation of 10 inorganic anions by suppressed IC using US EPA Method 300.1. The seven common anions (fluoride, chloride, bromide, nitrite, nitrate, sulfate, and phosphate), three oxyhalide anions (chlorite, chlorate, and bromate), and surrogate analyte anion (dichloroacetate) can be separated in one run.

VertiSep™ Anion AX300 column can also be used to determine inorganic anions in drinking water using US EPA Method 300.0 and trace bromate in drinking water using US EPA Method 317.0.

VertiSep PRP HPLC COLUMN
Separate Mono and Divalent Cations on Nonsuppressed or Suppressed IC
Separate Transition Metals without Post Column Reaction
Separate alkylamines
Compatible with Variety of Mobile Phases

       VertiSep™ Cation CX1 column is packed with hydrophilic copolymer with carbnoxyl functional groups providing higher pH stability on very low pH eluents over silica-based cation columns.

   VertiSep™ Cation CX1 Column separates Group I and Group II cations in one run under isocratic conditions. It can separates transition metals without post column reaction and UV dection. It can also separates various alkylamines such as methylamine, trimethylamine and triethyamine.

   VertiSep™ Cation CX1 Column is compatible with a variety of mobile phases including complexing acids (citric acid, tartaric acid, oxalic acid), mineral acids (nitric acid, hydrochloric acid, sulfuric acid) and noncomplexing organic acids (methanesulfonic acid).

 

VertiSep PRP HPLC COLUMN
Kromasil® Chiral AmyCoat™

Polysaccharide-based chiral HPLC phases for better performance
•The silica is based on an in-house developed matrix and coated with tris-(3,5-dimethylphenyl) carbamoyl amylose
•Better results and fast results
•High resolution with excellent selectivity.
•No pressure limits and stable performance
•Short equilibration time .
Vertical UPS HPLC COLUMN
Vertical UPS HPLC COLUMN
    Better results
        Kromasil AmyCoat with 3μm particle size enables a higher plate count and resolution for analytical chromatography. Combined with great selectivity, this facilitates the separation of the enantiomers.
    Fast results
        Not only you can get better results with Kromasil AmyCoat, you also get them faster. Thanks to a product characteristic like the absence of pressure limits, you can run analytical chromatography at very high flow rates and save time.
    Excellent selectivity
        The high selectivity and 3μm particles are also important factors in speeding up the method. You achieve an improved resolution, which, in turn, allows a higher flow rate.
    No pressure limits
        Kromasil AmyCoat withstands flow rates equivalent to pressures up to 400 bar which is about the limit for the HPLC system itself. This makes it possible to run analytical chromatography a lot faster.
    Stable performance

        From a stability perspective, you can run Kromasil AmyCoat using compatible normal, polar organic and reversed mobile phases. Switching between compatible normal to polar organic mobile phases will not lead to any degradation in performance, no need for solvent dedicated columns.

    Short equilibration time
        Column equilibration is a time-consuming activity when running chiral chromatography. Higher flow rates allow Kromasil AmyCoat to show substantially shorter equilibration times than other coated phases on the market.
        In general, long equilibration times are most pronounced when switching mobile phases containing basic additives to acidic additives or the other way around. The test with a Kromasil AmyCoatt 3 μm column switching between two compatible mobile phases shows how short equilibration times you can get. There are also two tests illustrating short equilibration times and additive switch for Kromasil AmyCoat with absolutely no sign of memory effects.
    Applications for AmyCoat
Vertical UPS HPLC COLUMN
        Typical applications include:
Acids,
Amines,
Alcohols,
Amides.
Kromasil® Chiral CelluCoat™
•Polysaccharide-based chiral HPLC phases for better performance
•The silica is based on an in-house developed matrix and coated with tris-(3,5-dimethylphenyl) carbamoyl cellulose
•Better results and fast results
•High resolution with excellent selectivity.
•No pressure limits and stable performance
•Short equilibration time .
Vertical UPS HPLC COLUMN
Vertical UPS HPLC COLUMN
    Better results
        Kromasil CelluCoat with 3μm particle size enables a higher plate count and resolution for analytical chromatography. Combined with great selectivity, this facilitates the separation of the enantiomers.
    Fast results
        Not only you can get better results with Kromasil CelluCoat, you also get them faster. Thanks to a product characteristic like the absence of pressure limits, you can run analytical chromatography at very high flow rates and save time.
    Excellent selectivity
        The high selectivity and 3μm particles are also important factors in speeding up the method. You achieve an improved resolution, which, in turn, allows a higher flow rate.
    No pressure limits
        Kromasil CelluCoat withstands flow rates equivalent to pressures up to 400 bar which is about the limit for the HPLC system itself. This makes it possible to run analytical chromatography a lot faster.
    Stable performance

        From a stability perspective, you can run Kromasil CelluCoat using compatible normal, polar organic and reversed mobile phases. Switching between compatible normal to polar organic mobile phases will not lead to any degradation in performance, no need for solvent dedicated columns.

    Short equilibration time
        Column equilibration is a time-consuming activity when running chiral chromatography. Higher flow rates allow Kromasil CelluCoat to show substantially shorter equilibration times than other coated phases on the market.
        In general, long equilibration times are most pronounced when switching mobile phases containing basic additives to acidic additives or the other way around. The test with a Kromasil CelluCoatt 3 μm column switching between two compatible mobile phases shows how short equilibration times you can get. There are also two tests illustrating short equilibration times and additive switch for Kromasil CelluCoat with absolutely no sign of memory effects.
    Applications for CelluCoat
Vertical UPS HPLC COLUMN
        Typical applications include:
Acids,
Amines,
Alcohols,
Amides,
Esters,
B-Lactones,
Lactones ,
Cyclic ketones ,
Alkaloids,
Dihydropyridines,
NSAIDs.
Protein-based chiral HPLC phases for better performance
A wide range of chiral applications
No deriveatization required
Aqueous mobile phase can be used in the separation
    Ultron ES-OVM is a chiral separation column immobilized with ovomucoid which is strong protein for denaturation. (US PATENT 6027648 Eisai Co., LTD.) that has numerous chiral recognition sites, making it applicable to a wide range of enantiomeric compounds.
This column recognizes hydrogen-bonding, polar, ionic, and hydrophobic sites, as well as the three-dimensional structure of sample molecules.
Ultron ES-OVM columns are compatible with aqueous and many of the commonly used organic solvents (for example, methanol, ethanol, and acetonitrile). Organic solvent concentrations greater than 50% are not recommended.
Typical applications include acidic and basic pharmaceuticals, pesticides and organic compounds.
Vertical UPS HPLC COLUMN
Cyclodextrin-based chiral HPLC phases for better performance
For the enantiomeric separation of hydrophobic cyclic compounds
Mobile phase of both reversed phase and normal phase can be used
Excellent stability and durability
    Ultron ES-CD and Ultron ES-PhCD are chemically bonded with Β-cyclodextrin (CD) and phenylcarbamated Β-cyclodextrin (PhCD), respectively.
Typical applications include acidic and basic pharmaceuticals, pesticides and organic compounds.
Vertical UPS HPLC COLUMN

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