PolyCAT A™ is a weak cation-exchange material (WCX) primarily used for protein variants involving deamidation, PEGylation, position of attachment, desialylation, etc., hemoglobin variant analysis, proteins with pI above 6.0, monoclonal antibodies
PolyHYDROXYETHYL A™ This neutral, polar material was developed specifically for Hydrophilic Interaction Chromatography (HILIC). It’s uses include analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules, peptide separations and mapping involving differences in polar groups, multidimensional purification of synthetic and natural peptides or fractionation of really complex digests, HPLC of solutes that aren’t soluble in aqueous media (membrane proteins;phospholipids), eliminating detergents, lipids, and salts from a sample, and oligonucleotides with their analogs.
In the absence of organic solvent, PolyHYDROXYETHYL A™ functions in the Size Exclusion Chromatography (SEC) mode.
PolySULFOETHYL A™ is a strong cation-exchange (SCX) material developed specifically for HPLC of peptides, it is a general-purpose alternative to reversed-phase (RPC), fractionating peptides by differences in charge rather than polarity. It used for multidimensional HPLC of peptide mixtures, such as tryptic digests in proteomics analyses (including iTRAQ® and ICAT®* reaction products), isolation of peptides from natural products, QC and purification of synthetic peptides, selective isolation of disulfide-linked peptides, phosphopeptides and C-terminal fragments from tryptic digests, and mapping of peptide digests (tryptic, V8, CNBr, etc.).
Hydrophobic Interaction Chromatography (HIC) Materials PolyEthyl A™, PolyMethyl A™,and PolyPropyl A™ are materials used to separate proteins on the basis of hydrophobic character, as does RPC. However, HIC uses totally aqueous buffers, retaining tertiary structure and biological activity. Selectivity is generally superior to that of RPC for proteins and polypeptides large enough to have significant secondary or tertiary structure. Use HIC for multidimensional protein purification, purification of polypeptides, characterization of antibodies, and QC analysis of proteins differing in the polarity of a single residue or modified positional variants.
PolyGLYCOPLEX A™ is neutral material that has the unusually high capacity needed to retain complex carbohydrates in the HILIC mode. Columns can frequently be operated with just acetonitrile and water, although charged carbohydrates may require inclusion of some salt such as ammonium acetate. more…
We offer a wide range of HPLC columns containing each of our chromatographic materials in a variety of lengths and diameters to best suite your needs.
TopTips™ and NuTips™
Lab-in-a-Tip is a new concept for sample cleanup, enzyme reactions, affinity chromatography, binding assays & many more applications.
Protect your column with the appropriate guard cartridge, we offer these in a variety of lengths to match each standard column diameter.
SPE cartridges are available in 10-packs of various styles and sizes containing each of our packing materials.
All of our chromatographic packing materials are available by the gram for purchase.
A guard cartridge of our SDS Removal Material, screwed into your RPC column, will successfully remove the SDS from peptide samples.
Electrostatic Repulsion – Hydrophilic Interaction Chromatography (ERLIC)
Protein Variant Analysis
Ion Exchange of Proteins with Organic Solvents
Oligonucleotides and PCR Products
Metabolomics, Amino Acids, and Polar Small Solutes
Hydrophilic Interaction Chromatography (HILIC)
Size Exclusion Chromatography (SEC)
Assay of Pathogenic Prion Proteins
Isolation of Proteins and Peptides from Extracts and Natural Products
Solid Phase Extraction